The IECS microscopy and imaging facility is open to both University of Regina researchers and external collaborators. A consultation and training session is required prior to use.
Regular wide-field fluorescence imaging is possible with the Zeiss Axio Observer Z1 inverted microscope. The fully motorized system is well suited for cell imaging and features Definite Focus to eliminate Z drift during long-term observations. In addition, a heated stage is available for temperature control of live specimens.
Currently one filter set is available (Zeiss 62 HE), whichis compatible with a wide range of dyes including for example DAPI, Alexa 350/405/430/488/568/594, Calcein, Cy 2, FITC, fluorescein, mCherry, MitoTracker Green, mPlum, mStrawberry, Nile Red, SYBR Green, and SYPRO Ruby.
Available objectives on the Zeiss Axio Observer Z1 are
Please contact Dr. Björn Wissel for information on specialized analyses and sample submissions.
For confocal and multi-photon imaging a Zeiss LSM 780 inverted confocal microscope with a tunable Coherent Chameleon laser is available. Available wavelengths are: 458, 488, 514, 543, 594 and a tunable laser from 680-1080 nm.
Please note that no dedicated UV laser is available. However, it is possible to utilize two-photon excitation at 700-820 nm (Em 455,478 nm) to visualize DAPI stains. For other dyes empirical optimization may be necessary.
Available objectives on the Zeiss LSM 780 are
Assisted imaging sessions are possible on a per case basis.